That is especially legitimate for immunohistochemistry by which ten% NBF has actually been proven to generally be a bad fixative (Fig. two). Some problems with the usage of 10% NBF for a fixative happen to be corrected by the introduction of other experimental tactics for instance antigen retrieval (Shi et al. 2001) and using small amplicons in RT-

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The incubation time in liquid paraffin really should be long adequate to obtain ample infiltration, although not any more than needed as a way to reduce extended exposure of the tissue to heat. For a similar rationale, it is suggested which the temperature in the liquid paraffin need to be not more than 2–four C earlier mentioned the paraffin's m

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